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KMID : 0378019740170010097
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New Medical Journal
1974 Volume.17 No. 1 p.97 ~ p.105
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Histologic Response of the Spleen in Rats Receiving a Purified Protein Antigen
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Abstract
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For this experiment, a total of 78 Sprague Dawley rats were divided into 5 groups. Groups 1 through 4 were consisted of 18 animals each, whereas the group 5 which was the control, was composed of 6 animals. Groups 1 and 2 were given a primary challenge of 5X10´ cells that were previously reacted in vitro with 10mg of ferritin. Group 3 and 4 received a primary injection of 1mg of ferritin. After the primary injection all animals were bled at weekly. intervals so that the antibody titer of individual animals could be monitored. After 5 weeks of the primary injection, 3 animals each from groups 1 through 4 and 1 animal from group 5 were sacrificed. These served day 0 animals of the histological materials. Into the remaining animals of groups 1 through 4, either 1mg of purified ferritin or 5X10´ cells previously reacted with 10mg ferritin were injected so that these groups ended in receiving the following combination of primary and secondary challenges After the secondary challenge, 3 animals each of groups 1 through 4 and 1 animal each of group 5 were sacrificed on day 1, 3, 7,15 or 30.
At the time of sacrifice, pieces of spleen was, removed and fixed in Bouin´s solution for 2 to 3 days. The samples were placed in 70 alcohol which was changed until the picric acid was removed. After alcoholic dehydration, the tissues were double-embedded in parlodion and paraffin, and the sections of 5 to 6 p in thickness were made on a rotary microtome. Six. sets of slides were made by mounting 4 these sections on a standard 1X3 microscope slide. Each set was stained with azure A and hematoxvlin.
The result were as follows:
1. Histologically, there was a marked increase in number of azurophilic cells of., the medullary cord and red pulp, as well as a similar increase in the germinal centers.
In animals that had received two injection of ferritin, there was a marked increase in number of azurophilic cells in red pulp when the large azurophilic cells were formed in germinal centers, the positional relationship of such cells to the central arteriole was of interest in that these large cells always occupied a proximal position to the central arteriole, whereas the rest of the germinal center.
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